Deposition of Polyphenols in Mare’s Milk Exosomes and Assessment of Their Bioavailability

Tatyana Sinyukova, Natalia Kavushevskaya, Anna Morozkina, Lyudmila Kovalenko

Sinyukova T., Kavushevskaya N., Morozkina A., Kovalenko L. Deposition of Polyphenols in Mare’s Milk Exosomes and Assessment of Their Bioavailability. Journal of Clinical Physiology and Pathology (JISCPP) 2024; 3 (1): 48—51

Polyphenols are a group of chemicals synthesized in plants (fruits, stems, roots, seeds). Polyphenols have geroprotective properties, but the use of polyphenols is limited due to their poor solubility in water. Milk-derived exosomes can be used as a transport system. The aim of the study was to evaluate the bioavailability of polyphenols in mare’s milk exosomes. Materials and methods: using isoelectric precipitation and ultracentrifugation, exosomes were made from mare’s milk. Quercetin was used as polyphenols. Encapsulation of quercetin in exosomes was carried out according to the method of encapsulation of medicinal substances in exosomes from cow’s milk. The capacity and efficiency of loading exosomes with antioxidants were evaluated. The release of quercetin from exosomes in vitro was carried out by equilibrium dialysis, then the percentage of binding in each time interval was calculated. Results: when studying the stability of the preservation of antioxidants in exosomes, we obtained a relative ratio (mV) for the quercitin standard — 69.4; for quercetin in exosomes immediately after inclusion — 90.1; for quercetin on the 12th day after storage at -80 ° C — 90.4. The efficiency of loading exosomes with quercetin was 55%. The proportion of quercetin released in total for 72 hours was 67.3%. Conclusion: The stability of quercetin in exosomes was confirmed for 12 days when stored at -80 °C. The results obtained during the dissociation of quercitin from exosomes in vitro using the equilibrium dialysis method indicate a gradual course of the release process.

polyphenols, quercetin, exosomes, bioavailability of polyphenols.